hsd17ß1 (Novus Biologicals)
Structured Review

Hsd17ß1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hsd17ß1/product/Novus Biologicals
Average 92 stars, based on 2 article reviews
Images
1) Product Images from "Capillary Western Immunoassay Optimization of Estrogen Related Factors in Human Subcutaneous Adipose Tissue"
Article Title: Capillary Western Immunoassay Optimization of Estrogen Related Factors in Human Subcutaneous Adipose Tissue
Journal: Methods and Protocols
doi: 10.3390/mps5020034
Figure Legend Snippet: All electropherograms report lysate concentrations of 0.5 and 1.0 mg/mL with 1:10 and 1:50 antibody concentrations. These detection antibodies were not close to their target protein’s predicted molecular weight and were, thus, not considered to be specifically binding target proteins of interest: ( A ) HSD17ß1 ( B ) HSD17ß2 ( C ) HSD17ß4 ( D ) HSD17ß7 and ( E ) SULTe1.
Techniques Used: Molecular Weight, Binding Assay
Figure Legend Snippet: All electropherograms report results from over-expresser lysates at concentrations of 0.5 and 0.8 mg/mL ( A – D ) except for SULTe1 which was tested at: 1/50, 1/100, 1/200, 1/400, 1/800. Empty vector negative controls are also displayed on each electropherogram. Lysates were paired with antibody concentrations of 1:10 and 1:50. Data are listed as follows: ( A ) HSD17ß1, ( B ) HSD17ß2, ( C ) HSD17ß4, ( D ) HSD17ß7, and ( E ) SULTe1.
Techniques Used: Plasmid Preparation
Figure Legend Snippet: All electropherograms report data for high fat (75 SAT lysate:25 over-expresser lysate) and low fat (25 SAT lysate:75 over-expresser lysate) mixtures with 1:10 antibody concentrations in duplicate: ( A ) HSD17ß1 ( B ) HSD17ß2 ( C ) HSD17ß4 and ( D ) SULTe1.
Techniques Used:
Figure Legend Snippet: Summary of expected and observed molecular weights for all 11 antibodies tested in each experiment.
Techniques Used: Extraction, Positive Control